ID   J01636; SV 1; linear; genomic DNA; STD; PRO; 7477 BP.
XX
AC   J01636; J01637; K01483; K01793;
XX
DT   30-NOV-1990 (Rel. 26, Created)
DT   09-SEP-2004 (Rel. 81, Last updated, Version 8)
XX
DE   E.coli lactose operon with lacI, lacZ, lacY and lacA genes.
XX
KW   acetyltransferase; beta-D-galactosidase; galactosidase; lac operon;
KW   lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene;
KW   lacZ gene; mutagenesis; palindrome; promoter region;
KW   thiogalactoside acetyltransferase.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RP   1243-1266
RX   PUBMED; 4587255.
RA   Gilbert W., Maxam A.;
RT   "The nucleotide sequence of the lac operator";
RL   Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973).
XX
RN   [2]
RP   1246-1308
RX   PUBMED; 4587256.
RA   Maizels N.M.;
RT   "The nucleotide sequence of the lactose messenger ribonucleic acid
RT   transcribed from the UV5 promoter mutant of Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 70(12):3585-3589(1973).
XX
RN   [3]
RX   PUBMED; 4598642.
RA   Gilbert W., Maizels N., Maxam A.;
RT   "Sequences of controlling regions of the lactose operon";
RL   Cold Spring Harb. Symp. Quant. Biol. 38:845-855(1974).
XX
RN   [4]
RA   Gilbert W., Gralla J., Majors A.J., Maxam A.;
RT   "Lactose operator sequences and the action of lac repressor";
RL   (in) Sund H., Blauer G. (Eds.);
RL   PROTEIN-LIGAND INTERACTIONS:193-207;
RL   Walter de Gruyter, New York (1975)
XX
RN   [5]
RP   1146-1282
RX   PUBMED; 1088926.
RA   Dickson R.C., Abelson J.N., Barnes W.M., Reznikoff W.S.;
RT   "Genetic regulation: the Lac control region.";
RL   Science 187(4171):27-35(1975).
XX
RN   [6]
RP   1227-1271
RA   Gilbert W., Maxam A., Mirzabekov A.;
RT   "Contacts between the lac repressor and DNA revealed by methylation";
RL   (in) Kjeldgaard N.C., Maaloe O. (Eds.);
RL   CONTROL OF RIBOSOME SYNTHESIS:138-143;
RL   Academic Press, New York (1976)
XX
RN   [7]
RX   DOI; 10.1038/260360a0.
RX   PUBMED; 768781.
RA   Marians K.J., Wu R.;
RT   "Structure of the lactose operator";
RL   Nature 260(5549):360-363(1976).
XX
RN   [8]
RP   1242-1268
RX   DOI; 10.1038/263748a0.
RX   PUBMED; 1069185.
RA   Heyneker H.L., Shine J., Goodman H.M, Boyer H.W., Rosenberg J.,
RA   Dickerson R.E., Narang S.A., Itakura K., Lin S., Riggs A.D.;
RT   "Synthetic lac operator is functional in vivo";
RL   Nature 263(5580):748-752(1976).
XX
RN   [9]
RX   DOI; 10.1016/S0022-2836(77)80132-0.
RX   PUBMED; 323498.
RA   Dickson R.C., Abelson J.N., Johnson P., Reznikoff W.S., Barnes W.M.;
RT   "Nucleotide sequence changes produced by mutations in the lac promoter of
RT   Escherichia coli";
RL   J. Mol. Biol. 111(1):65-75(1977).
XX
RN   [10]
RP   51-264
RX   PUBMED; 337294.
RA   Steege D.A.;
RT   "5'-Terminal nucleotide sequence of Escherichia coli lactose repressor
RT   mRNA: features of translational initiation and reinitiation sites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 74(10):4163-4167(1977).
XX
RN   [11]
RP   1-81
RX   DOI; 10.1038/274762a0.
RX   PUBMED; 355890.
RA   Calos M.P.;
RT   "DNA sequence for a low-level promoter of the lac repressor and an 'up'
RT   promoter mutation";
RL   Nature 274(5673):762-765(1978).
XX
RN   [12]
RP   49-1161
RX   DOI; 10.1038/274765a0.
RX   PUBMED; 355891.
RA   Farabaugh P.J.;
RT   "Sequence of the lacI gene";
RL   Nature 274(5673):765-769(1978).
XX
RN   [13]
RX   DOI; 10.1038/274770a0.
RX   PUBMED; 355892.
RA   Miller J.H., Coulondre C., Farabaugh P.J.;
RT   "Correlation of nonsense sites in the lacI gene with specific codons in the
RT   nucleotide sequence";
RL   Nature 274(5673):770-775(1978).
XX
RN   [14]
RX   DOI; 10.1007/BF00267233.
RX   PUBMED; 6770231.
RA   Calos M.P., Miller J.H.;
RT   "DNA sequence alteration resulting from a mutation impairing promoter
RT   function in the lac repressor gene";
RL   Mol. Gen. Genet. 178(1):225-227(1980).
XX
RN   [15]
RP   4306-5804
RX   DOI; 10.1038/283541a0.
RX   PUBMED; 6444453.
RA   Buechel D.E., Gronenborn B., Mueller-Hill B.;
RT   "Sequence of the lactose permease gene";
RL   Nature 283(5747):541-545(1980).
XX
RN   [16]
RX   PUBMED; 6271472.
RA   Miller J.H., Calos M.P., Galas D.J.;
RT   "Genetic and sequencing studies of the specificity of transposition into
RT   the lac region of E. coli";
RL   Cold Spring Harb. Symp. Quant. Biol. 45:243-257(1981).
XX
RN   [17]
RX   DOI; 10.1016/0014-5793(81)81076-9.
RX   PUBMED; 7023981.
RA   Chenchick A., Beabealashvilli R.S., Mirzabekov A.;
RT   "Topography of interaction of Escherichia coli RNA polymerase subunits with
RT   lac UV5 promoter";
RL   FEBS Lett. 128(1):46-50(1981).
XX
RN   [18]
RX   DOI; 10.1016/0378-1119(81)90038-X.
RX   PUBMED; 7016667.
RA   Betz J.L., Sadler J.R.;
RT   "Variants of a cloned synthetic lactose operator: I. A palindromic dimer
RT   lactose operator derived from one strand of the cloned 40-base pair
RT   operator";
RL   Gene 13(1):1-12(1981).
XX
RN   [19]
RX   DOI; 10.1016/0378-1119(81)90039-1.
RX   PUBMED; 6263752.
RA   Sadler J.R., Tecklenburg M.;
RT   "Cloning and characterization of the natural lactose operator";
RL   Gene 13(1):13-23(1981).
XX
RN   [20]
RX   DOI; 10.1016/0378-1119(81)90128-1.
RX   PUBMED; 6271642.
RA   Betz J.L., Sadler J.R.;
RT   "Variants of a cloned synthetic lactose operator: II.
RT   Chloramphenicol-resistant revertants retaining a lactose opeator in the CAT
RT   gene of plasmid pBR325";
RL   Gene 15(2-3):187-200(1981).
XX
RN   [21]
RX   DOI; 10.1007/BF00268783.
RX   PUBMED; 7038381.
RA   Calos M.P., Miller J.H.;
RT   "The DNA sequence change resulting from the I-Q1 mutation, which greatly
RT   increases promoter strength";
RL   Mol. Gen. Genet. 183(3):559-560(1981).
XX
RN   [22]
RX   PUBMED; 6278484.
RA   Mieschendahl M., Buechel D.E., Bocklage H., Mueller-Hill B.;
RT   "Mutations in the lacY gene of Escherichia coli define functional
RT   organization of lactose permease";
RL   Proc. Natl. Acad. Sci. U.S.A. 78(12):7652-7656(1981).
XX
RN   [23]
RX   DOI; 10.1016/0378-1119(82)90042-7.
RX   PUBMED; 6299890.
RA   Russell D.R., Bennett G.N.;
RT   "Construction and analysis of in vivo activity of E. coli promoter hybrids
RT   and promoter mutants that alter the -35 to -10 spacing";
RL   Gene 20(2):231-243(1982).
XX
RN   [24]
RX   PUBMED; 6288696.
RA   Horowitz H., Platt T.;
RT   "A termination site for LacI transcription is between the CAP site and the
RT   lac promoter.";
RL   J. Biol. Chem. 257(19):11740-11746(1982).
XX
RN   [25]
RX   PUBMED; 6290487.
RA   Klein R.D., Wells R.D.;
RT   "Effects of neighboring DNA homopolymers on the biochemical and physical
RT   properties of the Escherichia coli lactose promoter: I. Cloning and
RT   characterization studies";
RL   J. Biol. Chem. 257(21):12954-12961(1982).
XX
RN   [26]
RP   1183-1291
RX   PUBMED; 7041119.
RA   Weiher H., Schaller H.;
RT   "Segment-specific mutagenesis: extensive mutagenesis of a lac
RT   promoter/operator element.";
RL   Proc. Natl. Acad. Sci. U.S.A. 79(5):1408-1412(1982).
XX
RN   [27]
RX   PUBMED; 6305557.
RA   Van Dyke M.W., Dervan P.B.;
RT   "Footprinting with MPE-Fe(II). Complementary-strand analyses of distamycin-
RT   and actinomycin-binding sites on heterogeneous DNA";
RL   Cold Spring Harb. Symp. Quant. Biol. 47:347-353(1983).
XX
RN   [28]
RP   1287-4364
RX   PUBMED; 6313347.
RA   Kalnins A., Otto K., Ruether U., Mueller-Hill B.;
RT   "Sequence of the lacZ gene of Escherichia coli";
RL   EMBO J. 2(4):593-597(1983).
XX
RN   [29]
RX   PUBMED; 6309841.
RA   Cone K.C., Sellitti M.A., Steege D.A.;
RT   "Lac repressor mRNA transcription terminates in vivo in the lac control
RT   region";
RL   J. Biol. Chem. 258(18):11296-11304(1983).
XX
RN   [30]
RX   PUBMED; 6316325.
RA   Sadler J.R., Sasmor H., Betz J.L.;
RT   "A perfectly symmetric lac operator binds the lac repressor very tightly";
RL   Proc. Natl. Acad. Sci. U.S.A. 80(22):6785-6789(1983).
XX
RN   [31]
RX   PUBMED; 6582506.
RA   Glickman B.W., Ripley L.S.;
RT   "Structural intermediates of deletion mutagenesis: a role for palindromic
RT   DNA.";
RL   Proc. Natl. Acad. Sci. U.S.A. 81(2):512-516(1984).
XX
RN   [32]
RX   DOI; 10.1021/bi00332a019.
RX   PUBMED; 3896305.
RA   Spassky A., Kirkegaard K., Buc H.;
RT   "Changes in the DNA structure of the lac UV5 promoter during formation of
RT   an open complex with Escherichia coli RNA polymerase";
RL   Biochemistry 24(11):2723-2731(1985).
XX
RN   [33]
RX   DOI; 10.1016/0092-8674(85)90175-8.
RX   PUBMED; 2416465.
RA   Straney D.C., Crothers D.M.;
RT   "Intermediates in transcription initiation from the E. coli lac UV5
RT   promoter";
RL   Cell 43(2 Pt 1):449-459(1985).
XX
RN   [34]
RX   DOI; 10.1016/0378-1119(85)90267-7.
RX   PUBMED; 3932130.
RA   Looman A.C., de Gruyter M., Vogelaar A., van Knippenberg P.H.;
RT   "Effects of heterologous ribosomal binding sites on the transcription and
RT   translation of the lacZ gene of Escherichia coli";
RL   Gene 37(1-3):145-154(1985).
XX
RN   [35]
RX   PUBMED; 2999082.
RA   Mandecki W., Goldman R.A., Powell B.S., Caruthers M.H.;
RT   "lac Up-promoter mutants with increased homology to the consensus promoter
RT   sequence.";
RL   J. Bacteriol. 164(3):1353-1355(1985).
XX
RN   [36]
RX   DOI; 10.1016/0022-2836(85)90427-9.
RX   PUBMED; 2987506.
RA   Malamy M.H., Rahaim P.T., Hoffman C.S., Baghdoyan D., O'Connor M.B.,
RA   Miller J.F.;
RT   "A frameshift mutation at the junction of an IS1 insertion within lacZ
RT   restores beta-galactosidase activity via formation of an active lacZ-IS1
RT   fusion protein";
RL   J. Mol. Biol. 181(4):551-555(1985).
XX
RN   [37]
RP   5646-7477
RX   PUBMED; 3901000.
RA   Hediger M.A, Johnson D.F., Nierlich D.P., Zabin I.;
RT   "DNA sequence of the lactose operon: the lacA gene and the transcriptional
RT   termination region.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82(19):6414-6418(1985).
XX
CC   [3]  sites; UV5 mRNA transcripts and operator mutants. [(in)
CC   Sund,H. and Blauer,G. (eds.);Protein-Ligand Interactions:
CC   193-207;Walter de]  sites; operator mutational analysis. [7]
CC   sites; S1 and mung bean nuclease action on operator DNA. [9]
CC   sites; class I, II and III promoter mutant analysis. [13]  sites;
CC   lacI mutant analysis.
CC   [16]  sites; Tn5, Tn9 and Tn10 insertion sites in lac region. [14]
CC   sites; lacI promoter mutation UJ177.
CC   [18]  sites; palindromic dimer operator;.
CC   [19]  sites; natural operator sequence.
CC   [20]  sites; operator mutational analysis.
CC   [21]  sites; lacI-Q deletion.
CC   [17]  sites; RNA polymerase UV5 promoter interaction. [22]  sites;
CC   lacY mutational analysis.
CC   [24]  sites; lacI-promoted transcription termination. [25]  sites;
CC   wt and UV5 promoter sequence studies. [23]  sites; UV5 promoter
CC   mutational analysis.
CC   [30]  sites; perfectly symmetric operator sequence. [29]  sites;
CC   lacI mRNA termination site.
CC   [27]  sites; distamycin and actinomycin binding to promoter. [31]
CC   sites; lacI deletion studies.
CC   [35]  sites; promoter mutational studies.
CC   [33]  sites; DNAase I studies with promoter sequence. [34]  sites;
CC   ribosomal binding and translation initiation for lacZ. [36]  sites;
CC   insertion sequence IS1 integration in lacZ;. [32]  sites; DNAase I
CC   studies with promoter.
CC   [1] first reports a 27 bp operator(sites 1240-1266) with two-fold
CC   symmetries; the operator has also been defined to be bases
CC   1246-1266 or bases 1239-1273 [8]. [(in) Kjeldgaard,N.C. and Maaloe,
CC   O.(eds);Control of ribosome synthesis: 138-143;A] explores the
CC   ability of lac
CC   repressor protein to affect methylation of operator DNA.  [8]
CC   argues that DNA on both sides of the 21 bp operator (bases
CC   1246-1266) affects repressor binding but that the sequences of this
CC   DNA are probably not critical. [5] gives a larger sequence known as
CC   the promoter-operator region for the wild-type, whereas [2] and
CC   [26] give portions of this region for the mutant strain UV5. Within
CC   the promoter region, bases 1162-1199 are identified as the
CC   catabolite gene activator protein binding site (cap) and bases
CC   1200-1245 are the RNA polymerase interaction site. [10] reports a
CC   sequence for the 5'end of the lacI (repressor) gene and discusses
CC   restart in mutant strains. [11] presents a sequence for the lacI
CC   promoter region and identifies an I-Q mutation which enhances lacI
CC   transcription approximately ten-fold. [12] gives a complete
CC   sequence
CC   for lacI which agrees with the known lac repressor sequence. [26]
CC   examines the promoter-operator region in the UV5 strain (lac109)
CC   and studies 23 mutant derivatives of this sequence. This sequence
CC   agrees with known protein sequences for the lacZ, lacY and lacA
CC   enzymes. [15] notes that the fMet codon is not present
CC   for lacA and suggests that the 'ttg' codon (5727-5729), which
CC   immediately precedes the mature N-terminal asparagine codon, is the
CC   start codon. The cds for lacZ, lacY and lacA are included on a
CC   single mRNA transcript.
CC   Complete source information:
CC   Escherichia coli DNA [1],[(in) Kjeldgaard,N.C. and Maaloe,O.(eds);
CC   Control of ribosome synthesis: 138-143;A],[8],[12],[26]; mRNA [2],
CC   [5],[10]; clone
CC   lambda-h80dlac DNA [11],[15]; clone puk217 [28]; pgm8 [37].
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..7477
FT                   /organism="Escherichia coli"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   variation       16
FT                   /note="c in wild-type; t in 'up' promoter mutant I-Q [11]"
FT   mRNA            51..1230
FT                   /note="lacI (repressor) mRNA; preferred in vivo 3' end
FT                   [12],[29]"
FT   CDS             79..1161
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="lacI"
FT                   /note="lac repressor protein (gtg start codon)"
FT                   /db_xref="GOA:P03023"
FT                   /db_xref="PDB:1CJG"
FT                   /db_xref="PDB:1EFA"
FT                   /db_xref="PDB:1JWL"
FT                   /db_xref="PDB:1JYE"
FT                   /db_xref="PDB:1JYF"
FT                   /db_xref="PDB:1L1M"
FT                   /db_xref="PDB:1LBG"
FT                   /db_xref="PDB:1LBH"
FT                   /db_xref="PDB:1LBI"
FT                   /db_xref="PDB:1LCC"
FT                   /db_xref="PDB:1LCD"
FT                   /db_xref="PDB:1LQC"
FT                   /db_xref="PDB:1LTP"
FT                   /db_xref="PDB:1TLF"
FT                   /db_xref="PDB:2BJC"
FT                   /db_xref="UniProtKB/Swiss-Prot:P03023"
FT                   /protein_id="AAA24052.1"
FT                   /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
FT                   NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
FT                   EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
FT                   EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
FT                   MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
FT                   YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
FT                   ALADSLMQLARQVSRLESGQ"
FT   misc_signal     1162..1199
FT                   /note="cap protein binding site"
FT   variation       1183..1186
FT                   /note="ttag in wild-type; aatt in strain UV5 [26]"
FT   variation       1209..1211
FT                   /note="gct in wild-type; gt in mutant l305 [5]"
FT   variation       1212
FT                   /note="t in wild-type; a in mutant l241 [5]"
FT   variation       1230
FT                   /note="c in wild-type; a in mutant p-r-1a [5]"
FT   variation       1237..1238
FT                   /note="gt in wild-type; aa in strain UV5 [26]"
FT   variation       1242..1245
FT                   /note="gtgg in wild-type; ttca in synthetic operator [8]"
FT   misc_signal     1246..1266
FT                   /note="lac repressor protein binding site"
FT   mRNA            1246..>4358
FT                   /note="lacZ mRNA [2],[5]"
FT   variation       1267..1268
FT                   /note="tc in wild-type; tg in synthetic operator [8]"
FT   variation       1282..1291
FT                   /note="ctatgaccat in wild-type; gatccggcca in strain UV5
FT                   [26]"
FT   CDS             1284..4358
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="lacZ"
FT                   /note="beta-d-galactosidase"
FT                   /db_xref="GOA:P00722"
FT                   /db_xref="InterPro:IPR004199"
FT                   /db_xref="InterPro:IPR006101"
FT                   /db_xref="InterPro:IPR006102"
FT                   /db_xref="InterPro:IPR006103"
FT                   /db_xref="InterPro:IPR006104"
FT                   /db_xref="InterPro:IPR008979"
FT                   /db_xref="InterPro:IPR011013"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="InterPro:IPR013812"
FT                   /db_xref="PDB:1BGL"
FT                   /db_xref="PDB:1BGM"
FT                   /db_xref="PDB:1DP0"
FT                   /db_xref="PDB:1F49"
FT                   /db_xref="PDB:1F4A"
FT                   /db_xref="PDB:1F4H"
FT                   /db_xref="PDB:1GHO"
FT                   /db_xref="PDB:1HN1"
FT                   /db_xref="PDB:1JYN"
FT                   /db_xref="PDB:1JYV"
FT                   /db_xref="PDB:1JYW"
FT                   /db_xref="PDB:1JYX"
FT                   /db_xref="PDB:1JYY"
FT                   /db_xref="PDB:1JYZ"
FT                   /db_xref="PDB:1JZ0"
FT                   /db_xref="PDB:1JZ1"
FT                   /db_xref="PDB:1JZ2"
FT                   /db_xref="PDB:1JZ3"
FT                   /db_xref="PDB:1JZ4"
FT                   /db_xref="PDB:1JZ5"
FT                   /db_xref="PDB:1JZ6"
FT                   /db_xref="PDB:1JZ7"
FT                   /db_xref="PDB:1JZ8"
FT                   /db_xref="PDB:1PX3"
FT                   /db_xref="PDB:1PX4"
FT                   /db_xref="UniProtKB/Swiss-Prot:P00722"
FT                   /protein_id="AAA24053.1"
FT                   /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
FT                   DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
FT                   TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
FT                   QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
FT                   ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
FT                   ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
FT                   GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
FT                   YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
FT                   LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
FT                   KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
FT                   IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
FT                   TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
FT                   LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
FT                   NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
FT                   AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
FT                   EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
FT                   VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
FT                   FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
FT   CDS             4410..5663
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="lacY"
FT                   /note="lactose permease"
FT                   /db_xref="GOA:P02920"
FT                   /db_xref="InterPro:IPR000576"
FT                   /db_xref="InterPro:IPR007114"
FT                   /db_xref="PDB:1M2U"
FT                   /db_xref="PDB:1PV6"
FT                   /db_xref="PDB:1PV7"
FT                   /db_xref="PDB:2CFP"
FT                   /db_xref="PDB:2CFQ"
FT                   /db_xref="UniProtKB/Swiss-Prot:P02920"
FT                   /protein_id="AAA24054.1"
FT                   /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
FT                   GIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNIL
FT                   VGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFT
FT                   INNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKL
FT                   WFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPL
FT                   IINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQ
FT                   FEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFT
FT                   LSGPGPLSLLRRQVNEVA"
FT   CDS             5727..6338
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="lacA"
FT                   /note="thiogalactoside acetyltransferase (ttg start codon)"
FT                   /db_xref="GOA:P07464"
FT                   /db_xref="InterPro:IPR001451"
FT                   /db_xref="InterPro:IPR011004"
FT                   /db_xref="PDB:1KQA"
FT                   /db_xref="PDB:1KRR"
FT                   /db_xref="PDB:1KRU"
FT                   /db_xref="PDB:1KRV"
FT                   /db_xref="UniProtKB/Swiss-Prot:P07464"
FT                   /protein_id="AAA24055.1"
FT                   /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVE
FT                   KRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLI
FT                   APNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSI
FT                   VTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
XX
SQ   Sequence 7477 BP; 1739 A; 1991 C; 2004 G; 1743 T; 0 other;
     gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt        60
     caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt       120
     gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg       180
     cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa       240
     caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac       300
     gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg       360
     gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt       420
     ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt       480
     gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca       540
     cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg       600
     gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg       660
     cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg       720
     gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat       780
     gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg       840
     cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac       900
     gataccgaag acagctcatg ttatatcccg ccgtcaacca ccatcaaaca ggattttcgc       960
     ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag      1020
     ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg      1080
     caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc      1140
     cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc      1200
     accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata      1260
     acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac      1320
     aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc      1380
     ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc      1440
     gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa      1500
     gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga      1560
     tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc      1620
     cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa      1680
     gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc      1740
     tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg      1800
     acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga      1860
     gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg      1920
     tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta      1980
     atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg      2040
     actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg      2100
     cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac      2160
     gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg      2220
     tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt      2280
     tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc      2340
     gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga      2400
     tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt      2460
     atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg      2520
     aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct      2580
     ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga      2640
     gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt      2700
     atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg      2760
     acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct      2820
     tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc      2880
     cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat      2940
     actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg      3000
     atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt      3060
     ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca      3120
     cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat      3180
     ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc      3240
     tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg      3300
     tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg      3360
     ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg      3420
     ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg      3480
     ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg      3540
     gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg      3600
     ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg      3660
     acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg      3720
     cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg      3780
     atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca      3840
     gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag      3900
     tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag      3960
     cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg      4020
     ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc      4080
     cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc      4140
     gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc      4200
     atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg      4260
     ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg      4320
     gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc      4380
     tgcccgtatt tcgcgtaagg aaatccatta tgtactattt aaaaaacaca aacttttgga      4440
     tgttcggttt attctttttc ttttactttt ttatcatggg agcctacttc ccgtttttcc      4500
     cgatttggct acatgacatc aaccatatca gcaaaagtga tacgggtatt atttttgccg      4560
     ctatttctct gttctcgcta ttattccaac cgctgtttgg tctgctttct gacaaactcg      4620
     ggctgcgcaa atacctgctg tggattatta ccggcatgtt agtgatgttt gcgccgttct      4680
     ttatttttat cttcgggcca ctgttacaat acaacatttt agtaggatcg attgttggtg      4740
     gtatttatct aggcttttgt tttaacgccg gtgcgccagc agtagaggca tttattgaga      4800
     aagtcagccg tcgcagtaat ttcgaatttg gtcgcgcgcg gatgtttggc tgtgttggct      4860
     gggcgctgtg tgcctcgatt gtcggcatca tgttcaccat caataatcag tttgttttct      4920
     ggctgggctc tggctgtgca ctcatcctcg ccgttttact ctttttcgcc aaaacggatg      4980
     cgccctcttc tgccacggtt gccaatgcgg taggtgccaa ccattcggca tttagcctta      5040
     agctggcact ggaactgttc agacagccaa aactgtggtt tttgtcactg tatgttattg      5100
     gcgtttcctg cacctacgat gtttttgacc aacagtttgc taatttcttt acttcgttct      5160
     ttgctaccgg tgaacagggt acgcgggtat ttggctacgt aacgacaatg ggcgaattac      5220
     ttaacgcctc gattatgttc tttgcgccac tgatcattaa tcgcatcggt gggaaaaacg      5280
     ccctgctgct ggctggcact attatgtctg tacgtattat tggctcatcg ttcgccacct      5340
     cagcgctgga agtggttatt ctgaaaacgc tgcatatgtt tgaagtaccg ttcctgctgg      5400
     tgggctgctt taaatatatt accagccagt ttgaagtgcg tttttcagcg acgatttatc      5460
     tggtctgttt ctgcttcttt aagcaactgg cgatgatttt tatgtctgta ctggcgggca      5520
     atatgtatga aagcatcggt ttccagggcg cttatctggt gctgggtctg gtggcgctgg      5580
     gcttcacctt aatttccgtg ttcacgctta gcggccccgg cccgctttcc ctgctgcgtc      5640
     gtcaggtgaa tgaagtcgct taagcaatca atgtcggatg cggcgcgacg cttatccgac      5700
     caacatatca taacggagtg atcgcattga acatgccaat gaccgaaaga ataagagcag      5760
     gcaagctatt taccgatatg tgcgaaggct taccggaaaa aagacttcgt gggaaaacgt      5820
     taatgtatga gtttaatcac tcgcatccat cagaagttga aaaaagagaa agcctgatta      5880
     aagaaatgtt tgccacggta ggggaaaacg cctgggtaga accgcctgtc tatttctctt      5940
     acggttccaa catccatata ggccgcaatt tttatgcaaa tttcaattta accattgtcg      6000
     atgactacac ggtaacaatc ggtgataacg tactgattgc acccaacgtt actctttccg      6060
     ttacgggaca ccctgtacac catgaattga gaaaaaacgg cgagatgtac tcttttccga      6120
     taacgattgg caataacgtc tggatcggaa gtcatgtggt tattaatcca ggcgtcacca      6180
     tcggggataa ttctgttatt ggcgcgggta gtatcgtcac aaaagacatt ccaccaaacg      6240
     tcgtggcggc tggcgttcct tgtcgggtta ttcgcgaaat aaacgaccgg gataagcact      6300
     attatttcaa agattataaa gttgaatcgt cagtttaaat tataaaaatt gcctgatacg      6360
     ctgcgcttat caggcctaca agttcagcga tctacattag ccgcatccgg catgaacaaa      6420
     gcgcaggaac aagcgtcgca tcatgcctct ttgacccaca gctgcggaaa acgtactggt      6480
     gcaaaacgca gggttatgat catcagccca acgacgcaca gcgcatgaaa tgcccagtcc      6540
     atcaggtaat tgccgctgat actacgcagc acgccagaaa accacggggc aagcccggcg      6600
     atgataaaac cgattccctg cataaacgcc accagcttgc cagcaatagc cggttgcaca      6660
     gagtgatcga gcgccagcag caaacagagc ggaaacgcgc cgcccagacc taacccacac      6720
     accatcgccc acaataccgg caattgcatc ggcagccaga taaagccgca gaaccccacc      6780
     agttgtaaca ccagcgccag cattaacagt ttgcgccgat cctgatggcg agccatagca      6840
     ggcatcagca aagctcctgc ggcttgccca agcgtcatca atgccagtaa ggaaccgctg      6900
     tactgcgcgc tggcaccaat ctcaatatag aaagcgggta accaggcaat caggctggcg      6960
     taaccgccgt taatcagacc gaagtaaaca cccagcgtcc acgcgcgggg agtgaatacc      7020
     acgcgaaccg gagtggttgt tgtcttgtgg gaagaggcga cctcgcgggc gctttgccac      7080
     caccaggcaa agagcgcaac aacggcaggc agcgccacca ggcgagtgtt tgataccagg      7140
     tttcgctatg ttgaactaac cagggcgtta tggcggcacc aagcccaccg ccgcccatca      7200
     gagccgcgga ccacagcccc atcaccagtg gcgtgcgctg ctgaaaccgc cgtttaatca      7260
     ccgaagcatc accgcctgaa tgatgccgat ccccacccca ccaagcagtg cgctgctaag      7320
     cagcagcgca ctttgcgggt aaagctcacg catcaatgca ccgacggcaa tcagcaacag      7380
     actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc      7440
     cagcccgccc atggtaacca ccggcagagc ggtcgac                               7477
//
ID   X51872; SV 1; linear; genomic DNA; STD; PRO; 1832 BP.
XX
AC   X51872;
XX
DT   17-APR-1990 (Rel. 23, Created)
DT   05-JUL-1999 (Rel. 60, Last updated, Version 5)
XX
DE   Escherichia coli lacA gene for thiogalactoside transacetylase
XX
KW   lac operon; lacA gene; lacY gene; thiogalactoside transacetylase.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RC   (1-1832)
RP   1-1832
RX   PUBMED; 3901000.
RA   Hediger M.A, Johnson D.F., Nierlich D.P., Zabin I.;
RT   "DNA sequence of the lactose operon: the lacA gene and the transcriptional
RT   termination region.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82(19):6414-6418(1985).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1832
FT                   /organism="Escherichia coli"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   CDS             <1..18
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="lacY gene product"
FT                   /protein_id="CAA36161.1"
FT                   /translation="VNEVA"
FT   CDS             82..693
FT                   /transl_table=11
FT                   /gene="lacA"
FT                   /product="thiogalactoside transacetylase"
FT                   /db_xref="GOA:P07464"
FT                   /db_xref="InterPro:IPR001451"
FT                   /db_xref="InterPro:IPR011004"
FT                   /db_xref="PDB:1KQA"
FT                   /db_xref="PDB:1KRR"
FT                   /db_xref="PDB:1KRU"
FT                   /db_xref="PDB:1KRV"
FT                   /db_xref="UniProtKB/Swiss-Prot:P07464"
FT                   /protein_id="CAA36162.1"
FT                   /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVE
FT                   KRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLI
FT                   APNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSI
FT                   VTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
XX
SQ   Sequence 1832 BP; 519 A; 510 C; 450 G; 353 T; 0 other;
     gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg cgacgcttat ccgaccaaca        60
     tatcataacg gagtgatcgc attgaacatg ccaatgaccg aaagaataag agcaggcaag       120
     ctatttaccg atatgtgcga aggcttaccg gaaaaaagac ttcgtgggaa aacgttaatg       180
     tatgagttta atcactcgca tccatcagaa gttgaaaaaa gagaaagcct gattaaagaa       240
     atgtttgcca cggtagggga aaacgcctgg gtagaaccgc ctgtctattt ctcttacggt       300
     tccaacatcc atataggccg caatttttat gcaaatttca atttaaccat tgtcgatgac       360
     tacacggtaa caatcggtga taacgtactg attgcaccca acgttactct ttccgttacg       420
     ggacaccctg tacaccatga attgagaaaa aacggcgaga tgtactcttt tccgataacg       480
     attggcaata acgtctggat cggaagtcat gtggttatta atccaggcgt caccatcggg       540
     gataattctg ttattggcgc gggtagtatc gtcacaaaag acattccacc aaacgtcgtg       600
     gcggctggcg ttccttgtcg ggttattcgc gaaataaacg accgggataa gcactattat       660
     ttcaaagatt ataaagttga atcgtcagtt taaattataa aaattgcctg atacgctgcg       720
     cttatcaggc ctacaagttc agcgatctac attagccgca tccggcatga acaaagcgca       780
     ggaacaagcg tcgcatcatg cctctttgac ccacagctgc ggaaaacgta ctggtgcaaa       840
     acgcagggtt atgatcatca gcccaacgac gcacagcgca tgaaatgccc agtccatcag       900
     gtaattgccg ctgatactac gcagcacgcc agaaaaccac ggggcaagcc cggcgatgat       960
     aaaaccgatt ccctgcataa acgccaccag cttgccagca atagccggtt gcacagagtg      1020
     atcgagcgcc agcagcaaac agagcggaaa cgcgccgccc agacctaacc cacacaccat      1080
     cgcccacaat accggcaatt gcatcggcag ccagataaag ccgcagaacc ccaccagttg      1140
     taacaccagc gccagcatta acagtttgcg ccgatcctga tggcgagcca tagcaggcat      1200
     cagcaaagct cctgcggctt gcccaagcgt catcaatgcc agtaaggaac cgctgtactg      1260
     cgcgctggca ccaatctcaa tatagaaagc gggtaaccag gcaatcaggc tggcgtaacc      1320
     gccgttaatc agaccgaagt aaacacccag cgtccacgcg cggggagtga ataccacgcg      1380
     aaccggagtg gttgttgtct tgtgggaaga ggcgacctcg cgggcgcttt gccaccacca      1440
     ggcaaagagc gcaacaacgg caggcagcgc caccaggcga gtgtttgata ccaggtttcg      1500
     ctatgttgaa ctaaccaggg cgttatggcg gcaccaagcc caccgccgcc catcagagcc      1560
     gcggaccaca gccccatcac cagtggcgtg cgctgctgaa accgccgttt aatcaccgaa      1620
     gcatcaccgc ctgaatgatg ccgatcccca ccccaccaag cagtgcgctg ctaagcagca      1680
     gcgcactttg cgggtaaagc tcacgcatca atgcaccgac ggcaatcagc aacagactga      1740
     tggcgacact gcgacgttcg ctgacatgct gatgaagcca gcttccggcc agcgccagcc      1800
     cgcccatggt aaccaccggc agagcggtcg ac                                    1832
//
ID   V00294; SV 1; linear; genomic DNA; STD; PRO; 1113 BP.
XX
AC   V00294;
XX
DT   09-JUN-1982 (Rel. 01, Created)
DT   10-FEB-1999 (Rel. 58, Last updated, Version 2)
XX
DE   E. coli laci gene (codes for the lac repressor).
XX
KW   DNA binding protein; repressor.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RP   1-1113
RX   DOI; 10.1038/274765a0.
RX   PUBMED; 355891.
RA   Farabaugh P.J.;
RT   "Sequence of the lacI gene";
RL   Nature 274(5673):765-769(1978).
XX
CC   KST ECO.LACI
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1113
FT                   /organism="Escherichia coli"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   CDS             31..1113
FT                   /transl_table=11
FT                   /note="reading frame"
FT                   /db_xref="GOA:P03023"
FT                   /db_xref="PDB:1CJG"
FT                   /db_xref="PDB:1EFA"
FT                   /db_xref="PDB:1JWL"
FT                   /db_xref="PDB:1JYE"
FT                   /db_xref="PDB:1JYF"
FT                   /db_xref="PDB:1L1M"
FT                   /db_xref="PDB:1LBG"
FT                   /db_xref="PDB:1LBH"
FT                   /db_xref="PDB:1LBI"
FT                   /db_xref="PDB:1LCC"
FT                   /db_xref="PDB:1LCD"
FT                   /db_xref="PDB:1LQC"
FT                   /db_xref="PDB:1LTP"
FT                   /db_xref="PDB:1TLF"
FT                   /db_xref="PDB:2BJC"
FT                   /db_xref="UniProtKB/Swiss-Prot:P03023"
FT                   /protein_id="CAA23569.1"
FT                   /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
FT                   NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
FT                   EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
FT                   EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
FT                   MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
FT                   YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
FT                   ALADSLMQLARQVSRLESGQ"
XX
SQ   Sequence 1113 BP; 249 A; 304 C; 322 G; 238 T; 0 other;
     ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca        60
     gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt       120
     tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac       180
     cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt       240
     ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg       300
     ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg       360
     gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac       420
     caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc       480
     tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc       540
     gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt       600
     tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt       660
     cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg       720
     caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg       780
     ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta       840
     gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac caccatcaaa       900
     caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc       960
     caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg      1020
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca      1080
     cgacaggttt cccgactgga aagcgggcag tga                                   1113
//
ID   V00295; SV 1; linear; genomic DNA; STD; PRO; 1500 BP.
XX
AC   V00295;
XX
DT   09-JUN-1982 (Rel. 01, Created)
DT   07-JUL-1995 (Rel. 44, Last updated, Version 4)
XX
DE   E. coli lacY gene (codes for lactose permease).
XX
KW   membrane protein.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RP   1-1500
RX   DOI; 10.1038/283541a0.
RX   PUBMED; 6444453.
RA   Buechel D.E., Gronenborn B., Mueller-Hill B.;
RT   "Sequence of the lactose permease gene";
RL   Nature 283(5747):541-545(1980).
XX
CC   lacZ is a beta-galactosidase and lacA is transacetylase.
CC   KST ECO.LACY
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1500
FT                   /organism="Escherichia coli"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   CDS             <1..54
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="reading frame (lacZ)"
FT                   /db_xref="GOA:P00722"
FT                   /db_xref="PDB:1BGL"
FT                   /db_xref="PDB:1BGM"
FT                   /db_xref="PDB:1DP0"
FT                   /db_xref="PDB:1F49"
FT                   /db_xref="PDB:1F4A"
FT                   /db_xref="PDB:1F4H"
FT                   /db_xref="PDB:1GHO"
FT                   /db_xref="PDB:1HN1"
FT                   /db_xref="PDB:1JYN"
FT                   /db_xref="PDB:1JYV"
FT                   /db_xref="PDB:1JYW"
FT                   /db_xref="PDB:1JYX"
FT                   /db_xref="PDB:1JYY"
FT                   /db_xref="PDB:1JYZ"
FT                   /db_xref="PDB:1JZ0"
FT                   /db_xref="PDB:1JZ1"
FT                   /db_xref="PDB:1JZ2"
FT                   /db_xref="PDB:1JZ3"
FT                   /db_xref="PDB:1JZ4"
FT                   /db_xref="PDB:1JZ5"
FT                   /db_xref="PDB:1JZ6"
FT                   /db_xref="PDB:1JZ7"
FT                   /db_xref="PDB:1JZ8"
FT                   /db_xref="PDB:1PX3"
FT                   /db_xref="PDB:1PX4"
FT                   /db_xref="UniProtKB/Swiss-Prot:P00722"
FT                   /protein_id="CAA23570.1"
FT                   /translation="FQLSAGRYHYQLVWCQK"
FT   CDS             106..1359
FT                   /transl_table=11
FT                   /note="reading frame (lacY)"
FT                   /db_xref="GOA:P02920"
FT                   /db_xref="InterPro:IPR000576"
FT                   /db_xref="InterPro:IPR007114"
FT                   /db_xref="PDB:1M2U"
FT                   /db_xref="PDB:1PV6"
FT                   /db_xref="PDB:1PV7"
FT                   /db_xref="PDB:2CFP"
FT                   /db_xref="PDB:2CFQ"
FT                   /db_xref="UniProtKB/Swiss-Prot:P02920"
FT                   /protein_id="CAA23571.1"
FT                   /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
FT                   GIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNIL
FT                   VGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFT
FT                   INNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKL
FT                   WFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPL
FT                   IINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQ
FT                   FEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFT
FT                   LSGPGPLSLLRRQVNEVA"
FT   CDS             1423..>1500
FT                   /transl_table=11
FT                   /note="reading frame (lacA)"
FT                   /db_xref="GOA:P07464"
FT                   /db_xref="PDB:1KQA"
FT                   /db_xref="PDB:1KRR"
FT                   /db_xref="PDB:1KRU"
FT                   /db_xref="PDB:1KRV"
FT                   /db_xref="UniProtKB/Swiss-Prot:P07464"
FT                   /protein_id="CAA23572.1"
FT                   /translation="MNMPMTERIRAGKLFTDMCEGLPEKR"
XX
SQ   Sequence 1500 BP; 315 A; 342 C; 357 G; 486 T; 0 other;
     ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa        60
     ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa       120
     aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc       180
     tacttcccgt ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg       240
     ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg       300
     ctttctgaca aactcgggct gcgcaaatac ctgctgtgga ttattaccgg catgttagtg       360
     atgtttgcgc cgttctttat ttttatcttc gggccactgt tacaatacaa cattttagta       420
     ggatcgattg ttggtggtat ttatctaggc ttttgtttta acgccggtgc gccagcagta       480
     gaggcattta ttgagaaagt cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg       540
     tttggctgtg ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat       600
     aatcagtttg ttttctggct gggctctggc tgtgcactca tcctcgccgt tttactcttt       660
     ttcgccaaaa cggatgcgcc ctcttctgcc acggttgcca atgcggtagg tgccaaccat       720
     tcggcattta gccttaagct ggcactggaa ctgttcagac agccaaaact gtggtttttg       780
     tcactgtatg ttattggcgt ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat       840
     ttctttactt cgttctttgc taccggtgaa cagggtacgc gggtatttgg ctacgtaacg       900
     acaatgggcg aattacttaa cgcctcgatt atgttctttg cgccactgat cattaatcgc       960
     atcggtggga aaaacgccct gctgctggct ggcactatta tgtctgtacg tattattggc      1020
     tcatcgttcg ccacctcagc gctggaagtg gttattctga aaacgctgca tatgtttgaa      1080
     gtaccgttcc tgctggtggg ctgctttaaa tatattacca gccagtttga agtgcgtttt      1140
     tcagcgacga tttatctggt ctgtttctgc ttctttaagc aactggcgat gatttttatg      1200
     tctgtactgg cgggcaatat gtatgaaagc atcggtttcc agggcgctta tctggtgctg      1260
     ggtctggtgg cgctgggctt caccttaatt tccgtgttca cgcttagcgg ccccggcccg      1320
     ctttccctgc tgcgtcgtca ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc      1380
     gcgacgctta tccgaccaac atatcataac ggagtgatcg cattgaacat gccaatgacc      1440
     gaaagaataa gagcaggcaa gctatttacc gatatgtgcg aaggcttacc ggaaaaaaga      1500
//
ID   V00296; SV 1; linear; genomic DNA; STD; PRO; 3078 BP.
XX
AC   V00296;
XX
DT   13-JUL-1983 (Rel. 03, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 5)
XX
DE   E. coli gene lacZ coding for beta-galactosidase (EC 3.2.1.23).
XX
KW   galactosidase.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RP   1-3078
RX   PUBMED; 6313347.
RA   Kalnins A., Otto K., Ruether U., Mueller-Hill B.;
RT   "Sequence of the lacZ gene of Escherichia coli";
RL   EMBO J. 2(4):593-597(1983).
XX
RN   [2]
RX   PUBMED; 3038536.
RA   Zell R., Fritz H.J.;
RT   "DNA mismatch-repair in Escherichia coli counteracting the hydrolytic
RT   deamination of 5-methyl-cytosine residues";
RL   EMBO J. 6(6):1809-1815(1987).
XX
CC   Data kindly reviewed (18-MAY-1983) by U. Ruether
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..3078
FT                   /organism="Escherichia coli"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   CDS             <1..3072
FT                   /transl_table=11
FT                   /note="galactosidase"
FT                   /db_xref="GOA:P00722"
FT                   /db_xref="PDB:1BGL"
FT                   /db_xref="PDB:1BGM"
FT                   /db_xref="PDB:1DP0"
FT                   /db_xref="PDB:1F49"
FT                   /db_xref="PDB:1F4A"
FT                   /db_xref="PDB:1F4H"
FT                   /db_xref="PDB:1GHO"
FT                   /db_xref="PDB:1HN1"
FT                   /db_xref="PDB:1JYN"
FT                   /db_xref="PDB:1JYV"
FT                   /db_xref="PDB:1JYW"
FT                   /db_xref="PDB:1JYX"
FT                   /db_xref="PDB:1JYY"
FT                   /db_xref="PDB:1JYZ"
FT                   /db_xref="PDB:1JZ0"
FT                   /db_xref="PDB:1JZ1"
FT                   /db_xref="PDB:1JZ2"
FT                   /db_xref="PDB:1JZ3"
FT                   /db_xref="PDB:1JZ4"
FT                   /db_xref="PDB:1JZ5"
FT                   /db_xref="PDB:1JZ6"
FT                   /db_xref="PDB:1JZ7"
FT                   /db_xref="PDB:1JZ8"
FT                   /db_xref="PDB:1PX3"
FT                   /db_xref="PDB:1PX4"
FT                   /db_xref="UniProtKB/Swiss-Prot:P00722"
FT                   /protein_id="CAA23573.1"
FT                   /translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD
FT                   RPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT
FT                   YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQ
FT                   DSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
FT                   SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDE
FT                   RGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENG
FT                   LLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWY
FT                   TLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
FT                   GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPK
FT                   WSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI
FT                   KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQT
FT                   IEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQL
FT                   WLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGN
FT                   KRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKA
FT                   AGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE
FT                   VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYV
FT                   FPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGF
FT                   HMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
FT   mat_peptide     1..3069
XX
SQ   Sequence 3078 BP; 682 A; 841 C; 886 G; 669 T; 0 other;
     accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc        60
     gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa       120
     gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt       180
     gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag       240
     gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac       300
     accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg       360
     ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga       420
     attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt       480
     tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga       540
     gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag       600
     gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca       660
     caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg       720
     gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta       780
     tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat       840
     gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg       900
     tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc       960
     acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt      1020
     ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat      1080
     cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag      1140
     cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg      1200
     ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg      1260
     gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta      1320
     acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat      1380
     gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct      1440
     tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc      1500
     ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc      1560
     aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac      1620
     gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc      1680
     cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa      1740
     aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag      1800
     ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca      1860
     aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc      1920
     gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt      1980
     aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt      2040
     gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta      2100
     gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg      2160
     cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg      2220
     accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc      2280
     cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg      2340
     cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc      2400
     attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca      2460
     gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac      2520
     gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt      2580
     agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg      2640
     cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta      2700
     gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg      2760
     ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg      2820
     acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc      2880
     cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa      2940
     ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc      3000
     ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg      3060
     tgtcaaaaat aataataa                                                    3078
//
ID   V00307; SV 1; linear; genomic DNA; STD; PRO; 2271 BP.
XX
AC   V00307; J01654;
XX
DT   09-JUN-1982 (Rel. 01, Created)
DT   01-OCT-1996 (Rel. 49, Last updated, Version 8)
XX
DE   E. coli ompA gene (codes for the outer membrane protein II*).
XX
KW   membrane protein; unidentified reading frame.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RP   1-2271
RX   DOI; 10.1093/nar/8.13.3011.
RX   PUBMED; 6253901.
RA   Beck E., Bremer E.;
RT   "Nucleotide sequence of the gene ompA coding the outer membrane protein II*
RT   of Escherichia coli K-12";
RL   Nucleic Acids Res. 8(13):3011-3027(1980).
XX
RN   [2]
RP   783-1317
RX   PUBMED; 7001445.
RA   Movva N.R., Nakamura K., Inouye M.;
RT   "Regulatory region of the gene for the ompA protein, a major outer membrane
RT   protein of Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 77(7):3845-3849(1980).
XX
DR   UniProtKB/Swiss-Prot; P0A8N0; YCBG_ECOLI.
XX
CC   KST ECO.OMPA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2271
FT                   /organism="Escherichia coli"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   CDS             172..669
FT                   /transl_table=11
FT                   /note="reading frame (unknown protein)"
FT                   /db_xref="GOA:P0AFZ5"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0AFZ5"
FT                   /protein_id="CAA23587.1"
FT                   /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPMM
FT                   TQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVESMV
FT                   RALRTGNYSVVIGWLADDLTEEEHAEVVDAANEGNAMGFIIHSGKRILSRHETTFRAKN
FT                   SL"
FT   conflict        906..906
FT                   /note="may be missing [2]"
FT                   /citation=[2]
FT   CDS             1037..2077
FT                   /transl_table=11
FT                   /gene="ompA"
FT                   /product="ompA protein"
FT                   /db_xref="GOA:P0A910"
FT                   /db_xref="InterPro:IPR000498"
FT                   /db_xref="InterPro:IPR002368"
FT                   /db_xref="InterPro:IPR006664"
FT                   /db_xref="InterPro:IPR006665"
FT                   /db_xref="InterPro:IPR006690"
FT                   /db_xref="PDB:1BXW"
FT                   /db_xref="PDB:1G90"
FT                   /db_xref="PDB:1QJP"
FT                   /db_xref="PDB:2GE4"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0A910"
FT                   /protein_id="CAA23588.1"
FT                   /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFI
FT                   NNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKL
FT                   GYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQ
FT                   WTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVL
FT                   FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSV
FT                   VDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGIKDV
FT                   VTQPQA"
XX
SQ   Sequence 2271 BP; 567 A; 552 C; 548 G; 604 T; 0 other;
     gatccgttaa ctacgaaaat aggcaactta ttcttaaggg gcaagattaa tttatgtttt        60
     cccgtcacca acgacaaaat ttgcgaggct ctttccgaaa atagggttga tctttgttgt       120
     cactggatgt actgtacatc catacagtaa ctcacagggg ctggattgat tatgtacact       180
     tcaggctatg cacatcgttc ttcgtcgttc tcatccgcag caagtaaaat tgcgcgtgtc       240
     tctacggaaa acactacagc cgggcttatc agtgaagttg tctatcgcga agatcagccc       300
     atgatgacgc aacttctact gttgccattg ttacagcaac tcggtcagca atcgcgctgg       360
     caactctggt taacaccgca acaaaaactg agtcgggaat gggttcaggc atctgggcta       420
     cccttaacga aagtaatgca gattagccag ctctcccctt gccacactgt ggagtcaatg       480
     gttcgcgctt tacgcacggg caattacagt gtggtgatcg gttggttggc agatgatttg       540
     actgaagaag agcatgctga agttgttgat gcggcaaatg aaggtaacgc tatggggttt       600
     attatacatt ccggtaagcg catcctctca cgccacgaga caactttccg ggctaaaaat       660
     tcactctaat ttgtatcatt aagtaaattt aggattaatc ctggaacttt ttttgtcgcc       720
     cagccaatgc tttcagtcgt gactaatttc cttgcggagg cttgtctgag cggtttccgc       780
     gattctcttc tgtaaattgt cgctgacaaa aaagattaaa cataccttat acaagacttt       840
     tttttcatat gcctgacgga gttcacactt gtaagttttc aactacgttg tagactttac       900
     atcgccaagg gtgctcggca taagccgaag atatcggtag agttaatatt gagcagatcc       960
     cccggtgaag gatttaaccg tgttatctcg ttggagatat tcatggcgta ttttggatga      1020
     taacgaggcg caaaaaatga aaaagacagc tatcgcgatt gcagtggcac tggctggttt      1080
     cgctaccgta gcgcaggccg ctccgaaaga taacacctgg tacactggtg ctaaactggg      1140
     ctggtcccag taccatgata ctggtttcat caacaacaat ggcccgaccc atgaaaacca      1200
     actgggcgct ggtgcttttg gtggttacca ggttaacccg tatgttggct ttgaaatggg      1260
     ttacgactgg ttaggtcgta tgccgtacaa aggcagcgtt gaaaacggtg catacaaagc      1320
     tcagggcgtt caactgaccg ctaaactggg ttacccaatc actgacgacc tggacatcta      1380
     cactcgtctg ggtggcatgg tatggcgtgc agacactaaa tccaacgttt atggtaaaaa      1440
     ccacgacacc ggcgtttctc cggtcttcgc tggcggtgtt gagtacgcga tcactcctga      1500
     aatcgctacc cgtctggaat accagtggac gaacaacatc ggtgacgcac acaccatcgg      1560
     cactcgtccg gacaacggca tgctgagcct gggtgtttcc taccgtttcg gtcagggcga      1620
     ggcagctcca gtagttgctc cggctccagc tccggcaccg gaagtacaga ccaagcactt      1680
     cactctgaag tctgacgttc tgttcaactt caacaaagca accctgaaac cggaaggtca      1740
     ggctgctctg gatcagctgt acagccagct gagcaacttg gatccgaaag acggttccgt      1800
     agttgttctg ggttacaccg accgcatcgg ttctgacgct tacaaccagg gtctgtccga      1860
     gcgccgtgct cagtctgttg ttgattacct gatctccaaa ggtatcccgg cagacaagat      1920
     ctccgcacgt ggtatgggcg aatccaaccc ggttactggc aacacctgtg acaacgtgaa      1980
     acagcgtgct gcactgatcg actgcctggc tccggatcgt cgcgtagaga tcgaagttaa      2040
     aggtatcaaa gacgttgtaa ctcagccgca ggcttaagtt ctcgtctggt agaaaaacgc      2100
     tgctgcgggt ttttttttgc ctttagtaaa ttgaactgac tttcgtcagt tattccttac      2160
     ccagcaatgc ctgcagatcc tgcttcagag aagacatttt attcgcgtat ttctctttgt      2220
     tttccgcatc ttcaatcagc tgaacaatcg tttcagaaag cgttttaccg c               2271
//
ID   X77160; SV 1; linear; genomic DNA; STD; PRO; 1212 BP.
XX
AC   X77160;
XX
DT   13-APR-1994 (Rel. 39, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 8)
XX
DE   Pseudomonas aeruginosa (PAC1) amiB gene.
XX
KW   amiB gene.
XX
OS   Pseudomonas aeruginosa
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
XX
RN   [1]
RA   Wilson S.A., Wachira S.M., Drew R.E., Jones D., Pearl L.H.;
RT   "Identification of a putative ABC transporter in the Pseudomonas aeruginosa
RT   amidase operon";
RL   Unpublished.
XX
RN   [2]
RP   1-1212
RA   Wilson S.A.;
RT   ;
RL   Submitted (17-JAN-1994) to the EMBL/GenBank/DDBJ databases.
RL   S.A. Wilson, University College London, Dept of Biochemistry, Gower Street,
RL   London WC1E 6BT, UK
XX
RN   [3]
RP   1-1212
RX   DOI; 10.1074/jbc.270.32.18818.
RX   PUBMED; 7642533.
RA   Wilson S.A., Williams R.J., Pearl L.H., Drew R.E.;
RT   "Identification of two new genes in the Pseudomonas aeruginosa amidase
RT   operon, encoding an ATPase (AmiB) and a putative integral membrane protein
RT   (AmiS).";
RL   J. Biol. Chem. 270(32):18818-18824(1995).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1212
FT                   /organism="Pseudomonas aeruginosa"
FT                   /strain="PAC1"
FT                   /mol_type="genomic DNA"
FT                   /clone="plasmid pAS20"
FT                   /db_xref="taxon:287"
FT   CDS             97..1212
FT                   /transl_table=11
FT                   /gene="amiB"
FT                   /db_xref="GOA:Q51416"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q51416"
FT                   /protein_id="CAA54405.1"
FT                   /translation="MPFLSDMLDQSRRQQDEEQALARENLAEASLLQAHLSHRSALHSR
FT                   FRFDPAAVMDCLRAEVLGQEPALQAVEDMLKVVRADIADPRRPLFSALFLGPTGVGKTE
FT                   IVRALARALHGDAEGFCRVDMNTLSQEHYAAALTGAPPGYVGAKEGTTLLEQDKLDGSP
FT                   GRPGIVLFDELEKASPEVVHALLNVLDNGLLRVASGERTYHFRNTLVFMTSNLCAHEIQ
FT                   RYDERRQRLPWRLLPVGGERRRRDIDGMVRARLLKTFSPEFVNRLDSVVTFNWIERDVV
FT                   ARLVELEVQRLNRRLEKHRCRLEATPEVLAKIARAGFDRQFGARALRRSVRHHLEVPLA
FT                   EHLLDHHQPGDGNCTIYLASLEHERVRFVRR"
XX
SQ   Sequence 1212 BP; 193 A; 426 C; 403 G; 190 T; 0 other;
     gaaggaagcc tgacggcaga cgccgccagc ccggcgtgcc gtcgtgcggc acgccgtctc        60
     cgccatttcc cccctgtggc agaaggagtt tcatccatgc cttttctgag cgacatgctc       120
     gaccagtccc gccggcagca ggacgaggaa caggccctgg cgcgggagaa tcttgccgag       180
     gcaagtctgc tccaggccca cctgagtcac cgcagcgccc tgcacagccg tttccgtttc       240
     gacccggcgg cggtgatgga ctgcctgcgc gccgaggtgc tcggccagga accggcgcta       300
     caggccgtcg aggacatgct caaggtggtt cgcgcggaca tcgccgaccc gcgccgtccg       360
     ctgttcagcg cgctgttcct cggccccacc ggagtcggca agaccgagat cgtgcgcgcc       420
     ctggccaggg ccctgcacgg cgacgccgag gggttctgcc gggtggacat gaacaccctg       480
     tcccaggagc actatgccgc cgccctcacc ggtgcgccgc cgggctacgt cggggcgaag       540
     gagggcacca ccctgttgga gcaggacaag ctggacggca gtcccgggcg ccccggcatc       600
     gttctcttcg acgaactgga aaaggccagc ccggaagtgg tccatgcgtt gctcaacgta       660
     ctcgacaacg gcctgctacg ggtcgcttcc ggcgaacgca cctaccattt ccgcaacacc       720
     ctggtgttca tgaccagcaa tctctgcgcc catgagatcc agcgctacga cgagcgtcgc       780
     cagcgcctgc cctggcgcct gctgccggtc ggcggcgagc gccggcggcg ggacatcgac       840
     gggatggtcc gggcccggct gctgaagacc ttctcgccgg agttcgtcaa tcgtctcgat       900
     agcgtggtca ccttcaactg gatcgaacgc gacgtcgtcg cgcgcctggt cgagctggag       960
     gtgcagcggc tcaaccggcg cctggagaag catcgctgcc gcctggaggc gaccccggag      1020
     gtgctggcga agatcgcccg cgccggcttc gaccggcagt tcggcgcccg tgcgttgcgc      1080
     cgcagcgtcc gtcatcatct cgaggtaccg ctggccgagc atctgctcga tcaccaccag      1140
     ccgggcgacg ggaactgcac gatctacctg gcgagcctgg agcacgagcg ggttcgcttc      1200
     gtacggcgct ga                                                          1212
//
ID   M27612; SV 1; linear; genomic DNA; STD; PRO; 1065 BP.
XX
AC   M27612;
XX
DT   02-FEB-1990 (Rel. 22, Created)
DT   22-APR-2002 (Rel. 71, Last updated, Version 4)
XX
DE   Pseudomonas aeruginosa aliphatic amidase (amiE) gene, complete cds.
XX
KW   .
XX
OS   Pseudomonas aeruginosa
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
XX
RN   [1]
RP   1-1065
RX   DOI; 10.1016/0014-5793(87)80164-3.
RX   PUBMED; 3108030.
RA   Brammar W.J., Charles I.G., Matfield M., Liu C.P., Drew R.E., Clarke P.H.;
RT   "The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa";
RL   FEBS Lett. 215(2):291-294(1987).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1065
FT                   /organism="Pseudomonas aeruginosa"
FT                   /strain="PAC433"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:287"
FT   CDS             25..1065
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="amiE"
FT                   /product="aliphatic amidase"
FT                   /EC_number="3.5.1.4"
FT                   /db_xref="GOA:P11436"
FT                   /db_xref="PDB:1K17"
FT                   /db_xref="UniProtKB/Swiss-Prot:P11436"
FT                   /protein_id="AAA25697.1"
FT                   /translation="MRHGDISSSNDTVGVAVVNYKMPRLHTAAEVLDNARKIADMIVGM
FT                   KQGLPGMDLVVFPEYSLQGIMYDPAEMMETAVAIPGEETEIFSRACRKANVWGVFSLTG
FT                   ERHEEHPRKAPYNTLVLIDNNGEIVQKYRKIIPWCPIEGWYPGGQTYVSEGPKGMKISL
FT                   IICDDPNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVMMAKAMAWANNCYVAVANAAG
FT                   FDGVYSYFGHSAIIGFDGRTLGECGEEEMGIQYAQLSLSQIRDARANDQSQNHLFKILH
FT                   RGYSGLQASGDGDRGLAECPFEFYRTWVTDAEKARDNVERLTRSTTGVAQCPVGRLPYE
FT                   GLEKEA"
XX
SQ   Sequence 1065 BP; 220 A; 337 C; 343 G; 165 T; 0 other;
     aaataacaac aggaggtgat acccatgcgt cacggcgata tttccagcag caacgacacc        60
     gtcggagtgg ccgtggtcaa ctacaagatg ccgcgcctgc acaccgcggc ggaggtcctg       120
     gacaacgccc ggaagatcgc cgacatgatc gtcggcatga agcagggcct gcccggcatg       180
     gacctggtgg tgttcccgga gtacagcctg cagggcatca tgtacgatcc ggcggagatg       240
     atggaaaccg cggtggcgat ccccggcgag gaaaccgaga tattctcccg cgcctgccgc       300
     aaggccaacg tctggggcgt attctccctc accggcgaac ggcacgagga gcatccgcgc       360
     aaggcgccgt acaacaccct ggtgctgatc gacaacaacg gcgagatcgt ccagaagtac       420
     cgcaagatca ttccctggtg ccccatcgag ggctggtatc ccggtggcca gacctacgtc       480
     agcgaagggc cgaagggcat gaagatcagc ctgatcatct gcgacgaccc caattacccg       540
     gagatctggc gcgactgcgc gatgaagggc gccgagctga tcgtgcgctg ccagggctac       600
     atgtacccgg ccaaggacca gcaggtgatg atggccaagg ccatggcctg ggccaacaac       660
     tgctatgtgg cggtggccaa cgcggccggc ttcgacggtg tctattccta cttcggccac       720
     tcggcgatca tcggcttcga cggccgtacc ctcggtgagt gcggcgagga ggaaatgggt       780
     atccagtacg cccagctgtc cctttcgcag atccgcgatg cgcgcgccaa cgatcagtcg       840
     cagaaccacc tgttcaagat cctccaccgc ggctacagcg gcttgcaggc gtccggcgac       900
     ggcgaccggg gcctggcgga gtgtccgttc gagttctacc gcacctgggt caccgacgcc       960
     gagaaggcgc gcgacaatgt cgagcgactg acccgctcga ccaccggcgt ggcgcaatgc      1020
     ccggtcggcc ggctgcccta tgagggactg gagaaggaag cctga                      1065
//
ID   X13776; SV 1; linear; genomic DNA; STD; PRO; 2167 BP.
XX
AC   X13776; M43175;
XX
DT   19-APR-1989 (Rel. 19, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 24)
XX
DE   Pseudomonas aeruginosa amiC and amiR gene for aliphatic amidase regulation
XX
KW   aliphatic amidase regulator; amiC gene; amiR gene.
XX
OS   Pseudomonas aeruginosa
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
XX
RN   [1]
RP   1167-2167
RA   Rice P.M.;
RT   ;
RL   Submitted (16-DEC-1988) to the EMBL/GenBank/DDBJ databases.
RL   Rice P.M., EMBL, Postfach 10-2209, Meyerhofstrasse 1, 6900 Heidelberg, FRG.
XX
RN   [2]
RP   1167-2167
RX   DOI; 10.1016/0014-5793(89)80249-2.
RX   PUBMED; 2495988.
RA   Lowe N., Rice P.M., Drew R.E.;
RT   "Nucleotide sequence of the aliphatic amidase regulator gene of Pseudomonas
RT   aeruginosa";
RL   FEBS Lett. 246(1-2):39-43(1989).
XX
RN   [3]
RP   1-1292
RX   PUBMED; 1907262.
RA   Wilson S., Drew R.;
RT   "Cloning and DNA seqence of amiC, a new gene regulating expression of the
RT   Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC
RT   product.";
RL   J. Bacteriol. 173(16):4914-4921(1991).
XX
RN   [4]
RP   1-2167
RA   Rice P.M.;
RT   ;
RL   Submitted (04-SEP-1991) to the EMBL/GenBank/DDBJ databases.
RL   Rice P.M., EMBL, Postfach 10-2209, Meyerhofstrasse 1, 6900 Heidelberg, FRG.
XX
DR   GOA; Q51417.
DR   UniProtKB/Swiss-Prot; Q51417; AMIS_PSEAE.
XX
CC   Data kindly reviewed (04-SEP-1991) by Rice P. M.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2167
FT                   /organism="Pseudomonas aeruginosa"
FT                   /map="38 min"
FT                   /strain="PAC"
FT                   /isolate="PAC 1"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:287"
FT   CDS             1289..1879
FT                   /transl_table=11
FT                   /gene="amiR"
FT                   /note="aliphatic amidase regulator, positive  regulator of
FT                   amiE"
FT                   /db_xref="GOA:P10932"
FT                   /db_xref="PDB:1QO0"
FT                   /db_xref="UniProtKB/Swiss-Prot:P10932"
FT                   /protein_id="CAA32023.1"
FT                   /translation="MSANSLLGSLRELQVLVLNPPGEVSDALVLQLIRIGCSVRQCWPP
FT                   PEAFDVPVDVVFTSIFQNGHHDEIAALLAAGTPRTTLVALVEYESPAVLSQIIELECHG
FT                   VITQPLDAHRVLPVLVSARRISEEMAKLKQKTEQLQDRIAGQARINQAKVLLMQRHGWD
FT                   EREAHQHLSREAMKRREPILKIAQELLGNEPSA"
FT   CDS             135..1292
FT                   /transl_table=11
FT                   /gene="amiC"
FT                   /note="negative regulator of amiR"
FT                   /db_xref="GOA:P27017"
FT                   /db_xref="PDB:1PEA"
FT                   /db_xref="PDB:1QNL"
FT                   /db_xref="PDB:1QO0"
FT                   /db_xref="UniProtKB/Swiss-Prot:P27017"
FT                   /protein_id="CAA32024.1"
FT                   /translation="MGSHQERPLIGLLFSETGVTADIERSHAYGALLAVEQLNREGGVG
FT                   GRPIETLSQDPGGDPDRYRLCAEDFIRNRGVRFLVGCYMSHTRKAVMPVVERADALLCY
FT                   PTPYEGFEYSPNIVYGGPAPNQNSAPLAAYLIRHYGERVVFIGSDYIYPRESNHVMRHL
FT                   YRQHGGTVLEEIYIPLYPSDDDLQRAVERIYQARADVVFSTVVGTGTAELYRAIARRYG
FT                   DGRRPPIASLTTSEAEVAKMESDVAEGQVVVAPYFSSIDTPASRAFVQACHGFFPENAT
FT                   ITAWAEAAYWQTLLLGRAAQAAGNWRVEDVQRHLYDIDIDAPQGPVRVERQNNHSRLSS
FT                   RIAEIDARGVFQVRWQSPEPIRPDPYVVVHNLDDWSASMGGGPLP"
FT   promoter        8..24
FT                   /note="proposed rpoN-dependent promoter"
FT   promoter        65..81
FT                   /note="proposed rpoN-dependent promoter"
FT   RBS             121..126
FT                   /note="proposed Shine-Dalgarno sequence"
FT   variation       912..1167
FT                   /gene="amiC"
FT                   /replace=""
FT                   /note="ClaI fragment deleted in pSW36,  constitutive
FT                   phenotype"
FT   misc_feature    1
FT                   /note="last base of an XhoI site"
FT   misc_feature    648..653
FT                   /note="end of 658bp XhoI fragment, deletion in  pSW3 causes
FT                   constitutive expression of amiE"
FT   conflict        1281
FT                   /replace="g"
FT                   /citation=[3]
XX
SQ   Sequence 2167 BP; 363 A; 712 C; 730 G; 362 T; 0 other;
     ggtaccgctg gccgagcatc tgctcgatca ccaccagccg ggcgacggga actgcacgat        60
     ctacctggcg agcctggagc acgagcgggt tcgcttcgta cggcgctgag cgacagtcac       120
     aggagaggaa acggatggga tcgcaccagg agcggccgct gatcggcctg ctgttctccg       180
     aaaccggcgt caccgccgat atcgagcgct cgcacgcgta tggcgcattg ctcgcggtcg       240
     agcaactgaa ccgcgagggc ggcgtcggcg gtcgcccgat cgaaacgctg tcccaggacc       300
     ccggcggcga cccggaccgc tatcggctgt gcgccgagga cttcattcgc aaccgggggg       360
     tacggttcct cgtgggctgc tacatgtcgc acacgcgcaa ggcggtgatg ccggtggtcg       420
     agcgcgccga cgcgctgctc tgctacccga ccccctacga gggcttcgag tattcgccga       480
     acatcgtcta cggcggtccg gcgccgaacc agaacagtgc gccgctggcg gcgtacctga       540
     ttcgccacta cggcgagcgg gtggtgttca tcggctcgga ctacatctat ccgcgggaaa       600
     gcaaccatgt gatgcgccac ctgtatcgcc agcacggcgg cacggtgctc gaggaaatct       660
     acattccgct gtatccctcc gacgacgact tgcagcgcgc cgtcgagcgc atctaccagg       720
     cgcgcgccga cgtggtcttc tccaccgtgg tgggcaccgg caccgccgag ctgtatcgcg       780
     ccatcgcccg tcgctacggc gacggcaggc ggccgccgat cgccagcctg accaccagcg       840
     aggcggaggt ggcgaagatg gagagtgacg tggcagaggg gcaggtggtg gtcgcgcctt       900
     acttctccag catcgatacg cccgccagcc gggccttcgt ccaggcctgc catggtttct       960
     tcccggagaa cgcgaccatc accgcctggg ccgaggcggc ctactggcag accttgttgc      1020
     tcggccgcgc cgcgcaggcc gcaggcaact ggcgggtgga agacgtgcag cggcacctgt      1080
     acgacatcga catcgacgcg ccacaggggc cggtccgggt ggagcgccag aacaaccaca      1140
     gccgcctgtc ttcgcgcatc gcggaaatcg atgcgcgcgg cgtgttccag gtccgctggc      1200
     agtcgcccga accgattcgc cccgaccctt atgtcgtcgt gcataacctc gacgactggt      1260
     ccgccagcat gggcggggga ccgctcccat gagcgccaac tcgctgctcg gcagcctgcg      1320
     cgagttgcag gtgctggtcc tcaacccgcc gggggaggtc agcgacgccc tggtcttgca      1380
     gctgatccgc atcggttgtt cggtgcgcca gtgctggccg ccgccggaag ccttcgacgt      1440
     gccggtggac gtggtcttca ccagcatttt ccagaatggc caccacgacg agatcgctgc      1500
     gctgctcgcc gccgggactc cgcgcactac cctggtggcg ctggtggagt acgaaagccc      1560
     cgcggtgctc tcgcagatca tcgagctgga gtgccacggc gtgatcaccc agccgctcga      1620
     tgcccaccgg gtgctgcctg tgctggtatc ggcgcggcgc atcagcgagg aaatggcgaa      1680
     gctgaagcag aagaccgagc agctccagga ccgcatcgcc ggccaggccc ggatcaacca      1740
     ggccaaggtg ttgctgatgc agcgccatgg ctgggacgag cgcgaggcgc accagcacct      1800
     gtcgcgggaa gcgatgaagc ggcgcgagcc gatcctgaag atcgctcagg agttgctggg      1860
     aaacgagccg tccgcctgag cgatccgggc cgaccagaac aataacaaga ggggtatcgt      1920
     catcatgctg ggactggttc tgctgtacgt tggcgcggtg ctgtttctca atgccgtctg      1980
     gttgctgggc aagatcagcg gtcgggaggt ggcggtgatc aacttcctgg tcggcgtgct      2040
     gagcgcctgc gtcgcgttct acctgatctt ttccgcagca gccgggcagg gctcgctgaa      2100
     ggccggagcg ctgaccctgc tattcgcttt tacctatctg tgggtggccg ccaaccagtt      2160
     cctcgag                                                                2167
//
ID   X77161; SV 1; linear; genomic DNA; STD; PRO; 1130 BP.
XX
AC   X77161;
XX
DT   13-APR-1994 (Rel. 39, Created)
DT   31-JUL-2003 (Rel. 76, Last updated, Version 7)
XX
DE   Pseudomonas aeruginosa (PAC1) amiS gene.
XX
KW   amiS gene.
XX
OS   Pseudomonas aeruginosa
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
XX
RN   [1]
RA   Wilson S.A., Wachira S.M., Drew R.E., Jones D., Pearl L.H.;
RT   "Identification of a putative ABC transporter in the Pseudomonas aeruginosa
RT   amidase operon";
RL   Unpublished.
XX
RN   [2]
RP   1-1130
RA   Wilson S.A.;
RT   ;
RL   Submitted (17-JAN-1994) to the EMBL/GenBank/DDBJ databases.
RL   S.A. Wilson, University College London, Dept of Biochemistry, Gower Street,
RL   London WC1E 6BT, UK
XX
RN   [3]
RP   1-1130
RX   DOI; 10.1074/jbc.270.32.18818.
RX   PUBMED; 7642533.
RA   Wilson S.A., Williams R.J., Pearl L.H., Drew R.E.;
RT   "Identification of two new genes in the Pseudomonas aeruginosa amidase
RT   operon, encoding an ATPase (AmiB) and a putative integral membrane protein
RT   (AmiS).";
RL   J. Biol. Chem. 270(32):18818-18824(1995).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1130
FT                   /organism="Pseudomonas aeruginosa"
FT                   /strain="PAC1"
FT                   /mol_type="genomic DNA"
FT                   /clone="plasmid pAS20"
FT                   /db_xref="taxon:287"
FT   CDS             61..576
FT                   /transl_table=11
FT                   /gene="amiS"
FT                   /db_xref="GOA:Q51417"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q51417"
FT                   /protein_id="CAA54406.1"
FT                   /translation="MLGLVLLYVGAVLFLNAVWLLGKISGREVAVINFLVGVLSACVAF
FT                   YLIFSAAAGQGSLKAGALTLLFAFTYLWVAANQFLEVDGKGLGWFCLFVSLTACTVAIE
FT                   SFAGASGPFGLWNAVNWTVWALLWFCFFLLLGLSRGIQKPVAYLTLASAIFTAWLPGLL
FT                   LLGQVLKA"
XX
SQ   Sequence 1130 BP; 167 A; 335 C; 377 G; 251 T; 0 other;
     gagccgtccg cctgagcgat ccgggccgac cagaacaata acaagagggg tatcgtcatc        60
     atgctgggac tggttctgct gtacgttggc gcggtgctgt ttctcaatgc cgtctggttg       120
     ctgggcaaga tcagcggtcg ggaggtggcg gtgatcaact tcctggtcgg cgtgctgagc       180
     gcctgcgtcg cgttctacct gatcttttcc gcagcagccg ggcagggctc gctgaaggcc       240
     ggagcgctga ccctgctatt cgcttttacc tatctgtggg tggccgccaa ccagttcctc       300
     gaggtggacg gcaagggcct cggctggttc tgcctgttcg tcagcctcac cgcctgcacc       360
     gtggcgatcg agtcgttcgc cggcgccagt ggtccgttcg gcctgtggaa cgcggtcaac       420
     tggacagtct gggcgttgct ctggttctgt ttcttcctgc tgctggggct gtcccgcggc       480
     atccagaagc cggtggccta cctgaccctg gccagcgcca tattcaccgc ctggttgccc       540
     ggcctgctgc tgctcggaca ggtgctcaag gcatagcagg aagtcggaaa gggatgacgg       600
     cttgccgcca tcccgtccct tccgaacgcc tagccgagcg gccagttgat caccacgacg       660
     gcgtcgttgt agtcgttgtc ggtgccgtct tcagagccga ccagggcgaa gttcagctcg       720
     ttggtcagga ttacctgtgc cgagaccaga tccgaggggc ggccgttgac gctgacctgg       780
     acctgtacct tgccactgct gccggagttg agcacctggg tgccgatgac ggcgttattg       840
     gtgctttgcc cgctgaaggt cgcggccgtg ctcgttgttg accagcacgt tcaccgtctg       900
     ggttccggac gagttggcga aggcggtgac gccggaacct ggttgttggc gggaagggtg       960
     aacactcctt gtggttgcca tggtggtatc tccactgaat acctggcccc ttccttttca      1020
     ggcagccgtc tggcgcgcgg tatggcgtgt cgggagaaat ccgcagtcct tggcggcagg      1080
     cgatgcgcag gcaggaagga cgcatcgttc agccaatcta cgccgtcgac                 1130
//
